WebJan 20, 2010 · Synthetic glycoconjugates (GCs), which modulate cell glycosylation, increase the sensitivity of tumor cells to therapy or boost anti-cancer immune response. In the current study, we employed N-acetyl-D-glucosamine-calix[4]arene (GN4C) as a modulator of cell glycosylation of NK cells represented by the NK-92 cell line and … WebJul 9, 2016 · My laboratory research has focused on three major areas: (1) how aberrant glycosylation can influence cellular adhesion, trafficking and immune evasion in Multiple Myeloma (2) the role of the immune microenvironment in Multiple Myeloma and (3) the genetic modification of NK cells as cell therapy for cancer, including incorporation of …
Cells Free Full-Text Glycosylation of Immune Receptors in …
WebSep 15, 2008 · Glycosylation of the antibody Fc fragment is essential for Fc receptor–mediated activity. Carbohydrate heterogeneity is known to modulate the … birch and barley pullman dinner menu
Natural Killer Cell Activation Receptor NKp30 …
WebAug 22, 2016 · Tumor cells (target cells) were coincubated with PBMCs or NK cells (effector cells) at various effector/target (E/T) ratios in the presence or absence of sialidase or mAbs in triplicate. In a typical experiment, 100 µL of PBMCs or NK cells were added to a V-bottom 96-well plate containing 100 µL of target cells at 2 × 10 5 cells per ... WebActivated natural killer (NK) cells and T cells share many cytolytic components and surface antigens. ... a 38-residue external domain with multiple T-cell epitopes but no N-glycosylation sites or cysteine residues, and a 64-amino acid cytoplasmic domain with several phosphorylation sites. Northern blot analysis detected expression of a 0.9-kb ... WebJul 8, 2011 · De-N-glycosylation of NK cells results in decreased 2B4-mediated target cell lysis. A, the indicated NK cells were left untreated as a control or treated with 10 μg/ml of the glycosidase I and II inhibitor castanospermine for 24 h at 37 °C. 2B4 was immunoprecipitated and analyzed using SDS-PAGE and anti-2B4 Western blotting. dallas county license commissioner