Promofectin
WebJun 15, 2024 · CT-2000-RNA-50 - PromoFectin-siRNA - PromoFectin-siRNA, PromoKine - Each: Amazon.com: Industrial & Scientific WebPromofectin Huvec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > PromoCell > promofectin huvec. promofectin huvec transfection PromoCell is a verified supplier ...
Promofectin
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Webused the PromoFectin-siRNA cell transfection reagent from PromoKine GmbH to bring down the expression of MK2. All tested methods failed to knockdown MK2, even WebAug 16, 2024 · This PromoFectin derivative specifically targets cell surface receptors, leading to internalization of the complexed nucleic acids. Brief content visible, double tap to read full content. Full content visible, double tap to read brief content. Videos Help others learn more about this product by uploading a video! Upload video
WebFor optimal transfection conditions with PromoFectin-Hepatocyte, the cells should be 50-60% confluent. Typically, for transfection in 24-well plates, 50 000 hepatocytes are seeded per well, 24 hours before transfection. For primary hepatocytes, we recommend seeding 100 000 cells per well in 24-well plate two days before transfection and WebCell Transfection - PromoCell
WebApr 27, 2010 · PromoFectin® was from PromoCell (Heidelberg, Germany). Murine Anti-Xpress antiserum was obtained from Invitrogen (Carlsbad, CA) and horseradish peroxidase-conjugated anti-mouse antibody, raised in goat, was obtained from Sigma-Aldrich (Deisenhofen, Germany). 3,3´,5,5´- WebAfter fixation of transfected cells I observe strong Draq5 staining around the nucleus of my cells. It appears as small dots. The mycoplasma test was negative and I only see the …
WebTry to use non-lipid based transfection reagents, as may be your cells are not happy with the ones you have used, like FuGENE 6 or PromoFectin etc. 5. For KD experiments always take cells from...
WebPromoCell claims that PromoFectin-Hepatocyte reagent has been designed for transfection of cells expressing galactose-specific membrane lectins and has been very successfully … scotty\\u0027s flowers and giftsWebJun 15, 2024 · PromoFectin allows a very rapid and almost complete release of the nucleic acids into the cytosol. Moreover, it also favors DNA trafficking to the cell nucleus - a property that is particularly useful for transfection of slow-dividing or resting cells. Brief content visible, double tap to read full content. scotty\\u0027s food truck edmondsWebOct 30, 2014 · Human umbilical vein endothelial cells (HUVEC) were obtained from PromoCell, cultured in standard endothelial cell growth medium [ECGM; containing 0.1 ng/mL EGF and 1 ng/mL basic fibroblast growth factor (bFGF); PromoCell] and used at passage 1 to 10. scotty\\u0027s florist whittierWebThe most effective way for many primary cell types are usually the viral based methods usually adenovirus based (i.e.there is a kit called cytofect which has been optimised for hepatocytes). good... scotty\\u0027s flowersWebJul 18, 2015 · Another option is Promofectin, this is what I use in the lab. However best transfection efficiency up to 80% if you want to transfect high numbers of cells is the … scotty\\u0027s front street stage key westWebSpecialties: Promofect specializes in personalized apparel, screen printing and embroidery services. Our expert designers and promotional experts can help you choose the right product and apparel choice that will fit your … scotty\\u0027s fort myersWebOct 14, 2024 · The novel exchange protein activated by cyclic AMP (EPAC1) activator, I942, induces expression of the suppressor of cytokine signalling 3 (SOCS3) gene, thereby … scotty\\u0027s front street stage