Rpl22-ha
WebThe RiboTag mouse has a modified Ribosomal Protein L22 (RPL22), which is one of the 79 core proteins that constitute the ribosome particle as it is assembled in the nucleolus. The final coding exon 4 of the Rpl22 gene is … WebApr 6, 2024 · 在整体动物模型研究中，通过RiboTag小鼠与表达细胞类型特异性 Cre 重组酶的小鼠杂交，可获得细胞类型特异的HA标记核糖体亚单位蛋白Rpl22的基因修饰工具鼠，解剖分离特定组织进行TRAP-seq，可获得特定组织、特定细胞类型的翻译组数据。
Rpl22-ha
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WebApr 14, 2024 · Abstract. Antigen presentation is a fundamental component of cancer immunity. Cytotoxic CD8+ T cells, the primary mediators of cell killing, recognize peptide antigens presented in the context of Major Histocompatibility Complex Class I (MHC-I). MHC-I presents an astoundingly diverse array of peptides, collectively known as the … WebApr 13, 2024 · 在整体动物模型研究中，通过RiboTag小鼠与表达细胞类型特异性 Cre 重组酶的小鼠杂交，可获得细胞类型特异的HA标记核糖体亚单位蛋白Rpl22的基因修饰工具鼠，解剖分离特定组织进行TRAP-seq，可获得特定组织、特定细胞类型的翻译组数据。
WebFeb 24, 2024 · Rad21 with NexCre-dependent expression of an epitope-tagged ribosomal subunit (Rpl22-HA RiboTag; Ref. 39). We verified that NexCre-induced RPL22-HA expression was restricted to RAD21-depleted neurons (Supplementary Fig. 1a) and performed high throughput sequencing of Rpl22-HA RiboTag-associated mRNA (RiboTag RNA-seq). … WebSep 28, 2024 · b P6 Rpl22 HA/HA (control) and Pdgfb-iCre Rpl22 HA/HA retinas stained with Isolectin B4 (IB4, red) and anti-HA antibody (green). Scale bars represent 300 μm. c RT-qPCR analysis of transcripts ...
WebRPL22 ( a.k.a. EAP, HBP15, HBP15/L22, L22) Cloning Information Cloning method Gibson Cloning Terms and Licenses Academic/Nonprofit Terms UBMTA Ancillary Agreement for Plasmids Containing FP Materials Institut … WebSep 8, 2024 · RiboTag mouse carries an RPL22 subunit of the ribosome with a hemagglutinin tag at the C-terminal end (RPL22-HA), and its expression is induced by Cre recombinase (Sanz et al., 2009). Cell-type-specific expression of Cre recombinase thus enables isolation of total mRNA under translation in a cell-type-specific manner.
WebFeb 17, 2024 · Taking advantage of two Otp-specific reporter lines of transgenic mice (Otp-eGFP and Otp-Cre; Rpl22-HA), we identify and describe different Otp cell populations across various pallial regions, including the pallial amygdala, the piriform cortex, the mesocortex, the neocortex, and the hippocampal complex. Some of these populations can be ...
WebAug 8, 2009 · RPL22 ha Protein Is Incorporated into Polyribosomes. Having demonstrated that the RPL22 ha protein is stable, it was critical to demonstrate incorporation of RPL22 … ceramic cow salt and pepper shakersWebRpl22HA-expressing homozygous mice, which express only the RPL22HA protein, appear normal and are fertile. RPL22HA Protein Is Incorporated into Polyribosomes. Having dem … buy proxies speedWebMay 18, 2024 · In the RiboTag approach developed by McKnight and colleagues 4, cell-type-specific expression of Cre recombinase is used to activate expression of a hemagglutinin (HA) epitope-tagged ribosomal... buy proxies with btcWebMay 31, 2024 · After collecting the PBL, active mRNAs bound to the Rpl22 HA were isolated with anti-HA antibody-mediated ribosome pulldown and sequenced. (B) Immunohistochemistry of the PBL region of the Oprm1 Cre:Rpl22 HA mice with anti-GFP and anti-HA antibodies showed that Cre:GFP and Rpl22 HA were coexpressed in PBL Oprm1 … ceramic cows collectionWebDec 23, 2024 · In turn, Rpl22-HA protein expression was assessed by Western blotting, revealing high expression in intestinal and brain lysates of used mouse strains, whereas only small amounts were present in the spleen resulting either from Sox10 + astrocyte-like glia cells 25 or ChAT + T cells, 26 respectively. buy proxy for dating sitesWeb联系电话： 021-5992-3117 联系邮箱：[email protected] 联系地址：上海市嘉定区兴贤路1180号育成生物科技园 buy proxy cards mtgWeb2 days ago · In brief, RiboTag AAV (AAV2/5 GfaABC 1 D-Rpl22-HA or AAV1 hSYN1-Rpl22-HA) was injected into the dorsal striatum of adult C57BL/6NTac mice at 6 weeks of age. For RNA extraction from SAPAP3 KO mice ... buy proxifier