WebJan 11, 2012 · After analyzing biases in RNA sequence, secondary structure and RNA-adapter cofold structure, we conclude that T4 RNA ligases do not show significant primary sequence preference in RNA substrates, but are biased against structural features within RNAs and adapters. WebThe presence of Mg++ ion is required and the optimal concentration is 10mM. T4 DNA ligase has the unique ability to join sticky and blunt ended fragments. Cohesive end …
Spatiotemporally resolved transcriptomics reveals the subcellular …
WebLigation Protocol with T4 DNA Ligase (M0202) Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research … WebFAQ: Does PEG stimulate ligation efficiency? PEG 8000 greatly stimulates the ligation activity of T4 Rnl2tr K227Q. Maximum stimulation was observed at 15% (w/v) PEG 8000. PEG 4000 is also stimulatory with a similar concentration effect to PEG 8000. fuel filter head for duramax
Efficiency of T4 DNA ligase-catalyzed end joining after S1
WebT4 RNA ligases are commonly used to attach adapters to RNAs, but large differences in ligation efficiency make detection and quantitation problematic. We developed a ligation … WebEnzymatics’ high-concentration T4 DNA Ligase in combination with the 2X Rapid Ligation buffer greatly stimulates the rate and efficiency blunt-end ligation, therefore long incubations (>10 minutes) are NOT recommended and can greatly reduce the transformation efficiency of ligation products. WebBacteriophage T4 DNA ligase is a single polypeptide with a M.W of 68,000 Dalton requiring ATP as energy source. The maximal activity pH range is 7.5-8.0. The enzyme exhibits 40% of its activity at pH 6.9 and 65% at pH 8.3. The presence of Mg++ ion is required and the optimal concentration is 10mM. fuel filter housing 08925879